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Updated: Jul 18, 2026
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To be eligible to take the ASCP-MLT Certification Exam, candidates must have an associate degree in medical laboratory technology or a related field. They must also have completed an accredited medical laboratory technician program and have at least one year of clinical laboratory experience. ASCP-MLT exam is also open to candidates who have completed a bachelor's degree in a related field and have at least two years of clinical laboratory experience.
ASCP ASCP-MLT Certification Exam is an important milestone in any Medical Laboratory Technician’s career. Becoming certified through ASCP-MLT exam demonstrates a commitment to the field and enhances the technician’s knowledge and skills, ultimately contributing to the quality of patient care in the medical profession.
ASCP-MLT certification exam comprises various topics such as clinical chemistry, microbiology, hematology, immunohematology, and basic laboratory skills. Candidates who pass the exam have demonstrated their proficiency in the above subjects and other necessary skills such as record-keeping, working with laboratory instruments, managing laboratory safety, and interpretation of laboratory results. In general, the certification is also intended to verify the ability of the technician to work independently and manage their tasks with minimal supervision.
ASCP ASCP-MLT Exam Overview:
| Certification Vendor: | American Society for Clinical Pathology Board of Certification (ASCP BOC) |
| Exam Name: | Medical Laboratory Technician |
| Exam Number: | MLT(ASCP) |
| Exam Format: | Computer Adaptive Testing (CAT), Multiple-choice questions (one best answer) |
| Exam Price: | Application fee (USD 235–250, varies by region and international discounts) |
| Passing Score: | Scaled score of ≥ 400 (on 100–999 scale, CAT scoring) |
| Available Languages: | English |
| Real Exam Qty: | Approximately 100 |
| Related Certifications: | MLS(ASCP) Medical Laboratory Scientist PBT(ASCP) Phlebotomy Technician |
| Exam Duration: | 150 minutes |
| Certificate Validity Period: | Certification maintenance required (completion of ongoing continuing education every 3 years) |
| Sample Questions: | ASCP ASCP-MLT Sample Questions |
| Exam Way: | Computer-based testing delivered at Pearson VUE test centres |
| Pre Condition: | Completion of accredited MLT program or equivalent education/training OR relevant clinical experience following ASCP BOC eligibility routes |
| Official Syllabus URL: | https://www.ascp.org/boc/explore-credentials/view-all-credentials/MLT |
ASCP-MLT (Medical Laboratory Technician) exam is a certification exam offered by the American Society for Clinical Pathology (ASCP). It is designed to evaluate the knowledge and skills of aspiring medical laboratory technicians in the United States. Passing ASCP-MLT exam is a crucial step towards becoming a certified medical laboratory technician and pursuing a career in the field.
ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Blood Banking | 15–20% | - Blood products and components - Blood group systems and immunology - Transfusion practice and testing |
| Topic 2: Laboratory Operations | 5–10% | - Quality assessment and safety - Instrumentation and laboratory mathematics |
| Topic 3: Urinalysis and Other Body Fluids | 5–10% | - Physical and chemical testing - Microscopic analysis and disease correlation |
| Topic 4: Microbiology | 15–20% | - Virology and parasitology testing - Bacteriology and mycology procedures |
| Topic 5: Chemistry | 20–25% | - Electrolytes, acid-base, and toxicology - Carbohydrates, lipids, and enzymes |
| Topic 6: Hematology | 20–25% | - Physiology and disease states - Laboratory testing and hemostasis |
| Topic 7: Immunology | 5–10% | - Principles of immunology - Serologic procedures and immune diseases |
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